The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.
Components:The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium
Note: The serum- and xeno-free formulation means non-availability of attachment factors. Hence, coating with an appropriate adhesion factor is a pre-requisite. For the establishment of the culture conditions, it is recommended to test fibronectin (e.g. Human Fibronectin Solution (C-43060)) and vitronectin (e.g. Vitronectin (C-69201)) coating.
Key features:
- Suitable for long-term routine culture of adherently growing established human cancer cell lines
- Compatible with most commonly used human cancer cell lines
- Xeno-free and serum-free formulation
List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:
| Tissue | Tested Cell Lines | Cell Lines Origin | Remarks |
|---|---|---|---|
| Brain | BV2 | immortalized murine primary microglial cells | Coat with Fibronectin:1 µg/cm2 |
| Breast | MCF-7 | pleural effusion of metastatic human breast adenocarcinoma | Coat with Fibronectin:1 µg/cm2 |
| Colon | HT-29 | human colon adenocarcinoma | Coat with Vitronectin:0.5 µg/cm2 |
| Connective tissue | HT1080 | human fibrosarcoma | Coat with Fibronectin:1 µg/cm2 |
| Liver | HepG2 | hepatocellular carcinoma of the human liver | Coat with Vitronectin:0.5 µg/cm2 |
| Lung | A-549 | human lung carcinoma | Coat with Vitronectin:0.5 µg/cm2 |
| Prostate | LNCaP | lymph node metastasis of human prostate adenocarcinoma | 3D culture in C-28070 is recommended |
| Peripheral blood | MV-4-11 | Human acute myelogenous leukemia (suspension) | No coating required |
| Bone marrow | KG-1 | Human acute myelogenous leukemia (suspension) | No coating required |
| Kidney | ACHN | Human Renal Cell Carcinoma | Coat with Fibronectin:1 µg/cm2 |
| Brain | A172 | Human Glioblastoma | Coat with Fibronectin:1 µg/cm2 |
| Skeletal muscle | C2C12 | Mouse Myoblasts | Coat with Fibronectin:1 µg/cm2 |
| Skin | B16-F10 | Mouse Melanoma | Coat with Fibronectin:1 µg/cm2 |
| Abelson murine leukemia virus-induced tumor | RAW264.7 | Mouse Macrophage | Coat with Fibronectin:1 µg/cm2 |
| Brain, neuroectodermal | Neuro-2a | Mouse Neuroblastoma | Coat with Fibronectin:1 µg/cm2 |
